We will learn about 16S/ITS metagenomic analyses using QIIME2 and DADA2.
Upon successful completion of this module, you will be able to:
- MO 5.1. Compare and contrast OTUs and ASVs.
- MO 5.2. Discuss and critically evaluate each step of a 16S QIIME standard operating procedure (SOP).
- MO 5.3. Perform basic data analyses using QIIME and a downloaded dataset.
- MO 5.4. Apply tools from QIIME tutorial to analyses of other data sets:
- Create OTU tables to use for analyses in QIIME and elsewhere (e.g., R for plotting).
- Use alpha and beta diversity tools to assess richness and diversity within and between samples.
- Plot several different types of graphs using QIIME’s interface.
- MO. 5.5. Define and explain the concepts of metadata, OTU, rarefaction curve.
To complete this module, you will need to:
- Watch the Module 5 video and complete the review questions
- Annotate the third article using Persusall.
- Draft approach for the data analysis project.
- Meet with Digital Media Librarian for podcast recording.
Lab Entry for Labs 3 and 4
Your second lab entry is due tomorrow before lab. This lab entry is a summary of the Oxford Nanopore Technologies (ONT) 16S barcoding library prep sessions we did. The results will be analyzed in the next couple of weeks! BIT 477/577 Metagenomics Team