While our QIAGEN 16S/ITS Screening Libraries produced low yields, we submitted the amplified ITS sequences we prepared using the Zymo kit.
This happens! That’s how science goes. We may have had low PCR yields or issues with the PCR setup. Think of other reasons why we could get such low yields. What steps are common for all samples? I am attaching the TapeStation results for the samples you shared. Note that we don’t get the desired amplicons described on page 32 of the QIAGEN 16S/ITS Panel Handbook (opens in new window) Use this information in your lab entries.
Go back to the table of metadata and the concentrations you obtained and posted here (opens in new window) as you write your lab entries.
Discuss this in your lab entry and focus on the concentration values you obtained. Write a descriptive lab entry that is informative and accurate.
Some have not annotated the first article! Please do so and continue the conversations!
After watching the KBase tutorials and introductions to the Silver and Gold narratives, copy the narratives and run them! Learn what each step is doing and how you can use these tools to analyze whole-genome shotgun sequencing data.
This is not a setback; it is a learning opportunity. Next week, we will do Nanopore sequencing on some interesting samples!
CG
