Good morning, BIT 477/577 Metagenomics team:
We have an update from the Genomic Sciences Laboratory (GSL). They ran a PhiX (phage DNA) test run on the MiSeq, and it ran fine, which seems to indicate the error of the first run was most likely a library issue not an instrument issue. Illumina seems to think it may have over-clustered due to the presence of the mystery peak around 215 bp. Review the TapeStation results for our library attached here. What do you think? Post in the Student Help forum your ideas.
We appreciate your patience! This was certainly a learning experience for all. That band looked “ok” yet it caused some problems. I am glad we learned together and this wasn’t a critical sample.
We continue to grade the final projects. Thank you for checking the grade book. We will update BIT 577 course grades once and encourage you to calculate your grades manually to confirm Moodle. Thank you!
Have a great week!
The BIT Metagenomics team